pcr method Search Results


pcr-based method  (Federation of European Neuroscience Societies)
90
Federation of European Neuroscience Societies pcr-based method
Pcr Based Method, supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+method/pcr+based+method/pm12113931-24-2-14
Average 90 stars, based on 1 article reviews
pcr-based method - by Bioz Stars, 2026-09
90/100 stars
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book series rapid cycle real-time pcr  (Verlag GmbH)
90
Verlag GmbH book series rapid cycle real-time pcr
Book Series Rapid Cycle Real Time Pcr, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+method/rapid+cycle+real+time+pcr+methods+and+applications/10__1373_slash_clinchem__2004__037721-119-6-14
Average 90 stars, based on 1 article reviews
book series rapid cycle real-time pcr - by Bioz Stars, 2026-09
90/100 stars
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pcr protocols: a guide to methods and applications  (ACADEMIC PRESS INC)
90
ACADEMIC PRESS INC pcr protocols: a guide to methods and applications
Pcr Protocols: A Guide To Methods And Applications, supplied by ACADEMIC PRESS INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+method/pcr+protocols+a+guide+to+methods+and+applications/us08412460-1078-19-27
Average 90 stars, based on 1 article reviews
pcr protocols: a guide to methods and applications - by Bioz Stars, 2026-09
90/100 stars
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pcr method  (SinaClon BioScience)
90
SinaClon BioScience pcr method
Pcr Method, supplied by SinaClon BioScience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+method/pcr+method/pmc05894270-54-1-10
Average 90 stars, based on 1 article reviews
pcr method - by Bioz Stars, 2026-09
90/100 stars
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bcube 2.0 thermocycler  (Hyris Ltd)
90
Hyris Ltd bcube 2.0 thermocycler
Bcube 2.0 Thermocycler, supplied by Hyris Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+method/direct+qrt+pcr+method/pmc07513541-118-1-4
Average 90 stars, based on 1 article reviews
bcube 2.0 thermocycler - by Bioz Stars, 2026-09
90/100 stars
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real time (rt-) pcr method  (Lohmann Tierzucht)
90
Lohmann Tierzucht real time (rt-) pcr method
Real Time (Rt ) Pcr Method, supplied by Lohmann Tierzucht, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+method/real+time++rt+++pcr+method/pm28737107-58-28-18
Average 90 stars, based on 1 article reviews
real time (rt-) pcr method - by Bioz Stars, 2026-09
90/100 stars
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reference method rrt-pcr  (STAB VIDA)
90
STAB VIDA reference method rrt-pcr
Reference Method Rrt Pcr, supplied by STAB VIDA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+method/reference+method+rrt+pcr/pm35878801-98-8-5
Average 90 stars, based on 1 article reviews
reference method rrt-pcr - by Bioz Stars, 2026-09
90/100 stars
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pcr- fluorescence probing assay  (DaAn Gene)
90
DaAn Gene pcr- fluorescence probing assay
Pcr Fluorescence Probing Assay, supplied by DaAn Gene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+method/pcr+fluorescence+probe+method/pmc10257477-102-15-16
Average 90 stars, based on 1 article reviews
pcr- fluorescence probing assay - by Bioz Stars, 2026-09
90/100 stars
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c. trachomatis real-time taqman pcr method da0071  (DaAn Gene)
90
DaAn Gene c. trachomatis real-time taqman pcr method da0071
C . <t>trachomatis</t> -MCDA-AuNPs-LFB assay workflow. The workflow includes genomic DNA preparation, MCDA amplification, and AuNP-LFB visual interpretation, all completed within 40 min.
C. Trachomatis Real Time Taqman Pcr Method Da0071, supplied by DaAn Gene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+method/c++trachomatis+real+time+taqman+pcr+method+da0071/pmc09355032-79-22-28
Average 90 stars, based on 1 article reviews
c. trachomatis real-time taqman pcr method da0071 - by Bioz Stars, 2026-09
90/100 stars
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pcr methods appl  (Kunkel GmbH)
90
Kunkel GmbH pcr methods appl
C . <t>trachomatis</t> -MCDA-AuNPs-LFB assay workflow. The workflow includes genomic DNA preparation, MCDA amplification, and AuNP-LFB visual interpretation, all completed within 40 min.
Pcr Methods Appl, supplied by Kunkel GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+method/pcr+methods+appl/us09393319-331-21-12
Average 90 stars, based on 1 article reviews
pcr methods appl - by Bioz Stars, 2026-09
90/100 stars
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amplicon rescued multiplex polymerase chain reaction  (iRepertoire Inc)
90
iRepertoire Inc amplicon rescued multiplex polymerase chain reaction
C . <t>trachomatis</t> -MCDA-AuNPs-LFB assay workflow. The workflow includes genomic DNA preparation, MCDA amplification, and AuNP-LFB visual interpretation, all completed within 40 min.
Amplicon Rescued Multiplex Polymerase Chain Reaction, supplied by iRepertoire Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+method/amplicon+rescued+multiplex++arm++pcr+method/ppr0567456-324-30-39
Average 90 stars, based on 1 article reviews
amplicon rescued multiplex polymerase chain reaction - by Bioz Stars, 2026-09
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allele specific pcr method (as-pcr)  (Integragen sa)
90
Integragen sa allele specific pcr method (as-pcr)
C . <t>trachomatis</t> -MCDA-AuNPs-LFB assay workflow. The workflow includes genomic DNA preparation, MCDA amplification, and AuNP-LFB visual interpretation, all completed within 40 min.
Allele Specific Pcr Method (As Pcr), supplied by Integragen sa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+method/allele+specific+pcr+method++as+pcr+/10__4267_slash_2042_slash_61953-42-25-30
Average 90 stars, based on 1 article reviews
allele specific pcr method (as-pcr) - by Bioz Stars, 2026-09
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Image Search Results


C . trachomatis -MCDA-AuNPs-LFB assay workflow. The workflow includes genomic DNA preparation, MCDA amplification, and AuNP-LFB visual interpretation, all completed within 40 min.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Sensitive and visual identification of Chlamydia trachomatis using multiple cross displacement amplification integrated with a gold nanoparticle-based lateral flow biosensor for point-of-care use

doi: 10.3389/fcimb.2022.949514

Figure Lengend Snippet: C . trachomatis -MCDA-AuNPs-LFB assay workflow. The workflow includes genomic DNA preparation, MCDA amplification, and AuNP-LFB visual interpretation, all completed within 40 min.

Article Snippet: Using 135 suspected C. trachomatis -infected genital secretion samples from Hangzhou Women’s Hospital (Hangzhou, China), we compared our assay with a commercial C. trachomatis real-time TaqMan PCR method (DaAn Gene Co., Ltd. China) (Cat. #DA0071) on an Applied BiosystemsTM 7500 Real-Time PCR System (Life Technologies, Singapore), which was used as a reference method since it is commonly used in clinical Chinese laboratories, and its sensitivity was verified using C. trachomatis standard substance (Guangzhou BDS Biological Technology Co., Ltd.).

Techniques: Amplification

Schematic diagram showing AuNPs-LFB principles for the visual identification of C trachomatis -MCDA amplification products. (A) C trachomatis -MCDA amplification products (0.5 μl) and running buffer (100 μl) were simultaneously added to the sample pad. (B) Due to capillary action, the running buffer, containing (C) trachomatis -MCDA products, moved forward onto the conjugate pad and nitrocellulose (NC) membrane. Streptavidin-AuNPs were hydrated, rapidly released, and combined with C trachomatis -MCDA products at the conjugate pad. (C) FAM/biotin-labeled C trachomatis -MCDA products were arrested by anti-FAM at the TL strip, and streptavidin-DPNs were arrested at the biotin-BSA CL strip. (D) Interpretation of the C trachomatis -AuNP-LFB assay. For a positive result, both the CL and TL appeared on the biosensor. For a negative result, only the CL was observed on the AuNP-LFB. TL: test line; CL: control line.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Sensitive and visual identification of Chlamydia trachomatis using multiple cross displacement amplification integrated with a gold nanoparticle-based lateral flow biosensor for point-of-care use

doi: 10.3389/fcimb.2022.949514

Figure Lengend Snippet: Schematic diagram showing AuNPs-LFB principles for the visual identification of C trachomatis -MCDA amplification products. (A) C trachomatis -MCDA amplification products (0.5 μl) and running buffer (100 μl) were simultaneously added to the sample pad. (B) Due to capillary action, the running buffer, containing (C) trachomatis -MCDA products, moved forward onto the conjugate pad and nitrocellulose (NC) membrane. Streptavidin-AuNPs were hydrated, rapidly released, and combined with C trachomatis -MCDA products at the conjugate pad. (C) FAM/biotin-labeled C trachomatis -MCDA products were arrested by anti-FAM at the TL strip, and streptavidin-DPNs were arrested at the biotin-BSA CL strip. (D) Interpretation of the C trachomatis -AuNP-LFB assay. For a positive result, both the CL and TL appeared on the biosensor. For a negative result, only the CL was observed on the AuNP-LFB. TL: test line; CL: control line.

Article Snippet: Using 135 suspected C. trachomatis -infected genital secretion samples from Hangzhou Women’s Hospital (Hangzhou, China), we compared our assay with a commercial C. trachomatis real-time TaqMan PCR method (DaAn Gene Co., Ltd. China) (Cat. #DA0071) on an Applied BiosystemsTM 7500 Real-Time PCR System (Life Technologies, Singapore), which was used as a reference method since it is commonly used in clinical Chinese laboratories, and its sensitivity was verified using C. trachomatis standard substance (Guangzhou BDS Biological Technology Co., Ltd.).

Techniques: Amplification, Membrane, Labeling, Stripping Membranes, Control

C . trachomatis -MCDA-AuNPs-LFB degenerate primers used in this study.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Sensitive and visual identification of Chlamydia trachomatis using multiple cross displacement amplification integrated with a gold nanoparticle-based lateral flow biosensor for point-of-care use

doi: 10.3389/fcimb.2022.949514

Figure Lengend Snippet: C . trachomatis -MCDA-AuNPs-LFB degenerate primers used in this study.

Article Snippet: Using 135 suspected C. trachomatis -infected genital secretion samples from Hangzhou Women’s Hospital (Hangzhou, China), we compared our assay with a commercial C. trachomatis real-time TaqMan PCR method (DaAn Gene Co., Ltd. China) (Cat. #DA0071) on an Applied BiosystemsTM 7500 Real-Time PCR System (Life Technologies, Singapore), which was used as a reference method since it is commonly used in clinical Chinese laboratories, and its sensitivity was verified using C. trachomatis standard substance (Guangzhou BDS Biological Technology Co., Ltd.).

Techniques: Sequencing

Confirmation and verification of (C) trachomatis -MCDA products. C trachomatis -MCDA products were measured simultaneously using malachite green (MG) (A) and AuNPs-LFB (B) . Tube 1/Biosensor 1: positive result for C trachomatis ompA standard plasmids; Tube 2/Biosensor 2: negative result for Neisseria gonorrhoeae ; Tube 3/Biosensor 3: negative result for Ureaplasma urealyticum ; Tube 4/Biosensor 4: blank control (distilled water, DW). TL: test line; CL: control line.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Sensitive and visual identification of Chlamydia trachomatis using multiple cross displacement amplification integrated with a gold nanoparticle-based lateral flow biosensor for point-of-care use

doi: 10.3389/fcimb.2022.949514

Figure Lengend Snippet: Confirmation and verification of (C) trachomatis -MCDA products. C trachomatis -MCDA products were measured simultaneously using malachite green (MG) (A) and AuNPs-LFB (B) . Tube 1/Biosensor 1: positive result for C trachomatis ompA standard plasmids; Tube 2/Biosensor 2: negative result for Neisseria gonorrhoeae ; Tube 3/Biosensor 3: negative result for Ureaplasma urealyticum ; Tube 4/Biosensor 4: blank control (distilled water, DW). TL: test line; CL: control line.

Article Snippet: Using 135 suspected C. trachomatis -infected genital secretion samples from Hangzhou Women’s Hospital (Hangzhou, China), we compared our assay with a commercial C. trachomatis real-time TaqMan PCR method (DaAn Gene Co., Ltd. China) (Cat. #DA0071) on an Applied BiosystemsTM 7500 Real-Time PCR System (Life Technologies, Singapore), which was used as a reference method since it is commonly used in clinical Chinese laboratories, and its sensitivity was verified using C. trachomatis standard substance (Guangzhou BDS Biological Technology Co., Ltd.).

Techniques: Control

Optimizing the temperature for the C. trachomatis -MCDA assay. C. trachomatis -MCDA amplification of ompA was monitored using real-time turbidity. Corresponding amplicon concentration curves are marked in graphs. Turbidity > 0.1 indicated a positive value. (A–H) Eight kinetic graphs were generated at different temperatures (63°C–70°C at 1°C intervals) with C. trachomatis ompA -plasmids at 1 × 10 3 copies. Graph E (67°C) showed the fastest and most robust amplification.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Sensitive and visual identification of Chlamydia trachomatis using multiple cross displacement amplification integrated with a gold nanoparticle-based lateral flow biosensor for point-of-care use

doi: 10.3389/fcimb.2022.949514

Figure Lengend Snippet: Optimizing the temperature for the C. trachomatis -MCDA assay. C. trachomatis -MCDA amplification of ompA was monitored using real-time turbidity. Corresponding amplicon concentration curves are marked in graphs. Turbidity > 0.1 indicated a positive value. (A–H) Eight kinetic graphs were generated at different temperatures (63°C–70°C at 1°C intervals) with C. trachomatis ompA -plasmids at 1 × 10 3 copies. Graph E (67°C) showed the fastest and most robust amplification.

Article Snippet: Using 135 suspected C. trachomatis -infected genital secretion samples from Hangzhou Women’s Hospital (Hangzhou, China), we compared our assay with a commercial C. trachomatis real-time TaqMan PCR method (DaAn Gene Co., Ltd. China) (Cat. #DA0071) on an Applied BiosystemsTM 7500 Real-Time PCR System (Life Technologies, Singapore), which was used as a reference method since it is commonly used in clinical Chinese laboratories, and its sensitivity was verified using C. trachomatis standard substance (Guangzhou BDS Biological Technology Co., Ltd.).

Techniques: Amplification, Concentration Assay, Generated

Sensitivity analysis of C trachomatis -MCDA-AuNPs-LFB using C trachomatis ompA -plasmid serial dilutions. Serial dilutions (1.0 × 10 4 , 1.0 × 10 3 , 1.0 × 10 2 , 1.0 × 10 1 , 1.0 × 10 0 , and 1.0 × 10 −1 copies) of C trachomatis ompA -plasmids were used as templates, and distilled water (DW) was used as the negative control. Results were simultaneously analyzed by malachite green (MG) (A) and AuNPs-LFB (B) . The limit of detection (LoD) for C trachomatis -MCDA-AuNP-LFB was 10 copies/test. CL, control line; TL, test line.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Sensitive and visual identification of Chlamydia trachomatis using multiple cross displacement amplification integrated with a gold nanoparticle-based lateral flow biosensor for point-of-care use

doi: 10.3389/fcimb.2022.949514

Figure Lengend Snippet: Sensitivity analysis of C trachomatis -MCDA-AuNPs-LFB using C trachomatis ompA -plasmid serial dilutions. Serial dilutions (1.0 × 10 4 , 1.0 × 10 3 , 1.0 × 10 2 , 1.0 × 10 1 , 1.0 × 10 0 , and 1.0 × 10 −1 copies) of C trachomatis ompA -plasmids were used as templates, and distilled water (DW) was used as the negative control. Results were simultaneously analyzed by malachite green (MG) (A) and AuNPs-LFB (B) . The limit of detection (LoD) for C trachomatis -MCDA-AuNP-LFB was 10 copies/test. CL, control line; TL, test line.

Article Snippet: Using 135 suspected C. trachomatis -infected genital secretion samples from Hangzhou Women’s Hospital (Hangzhou, China), we compared our assay with a commercial C. trachomatis real-time TaqMan PCR method (DaAn Gene Co., Ltd. China) (Cat. #DA0071) on an Applied BiosystemsTM 7500 Real-Time PCR System (Life Technologies, Singapore), which was used as a reference method since it is commonly used in clinical Chinese laboratories, and its sensitivity was verified using C. trachomatis standard substance (Guangzhou BDS Biological Technology Co., Ltd.).

Techniques: Plasmid Preparation, Negative Control, Control

Optimal amplification time for the C. trachomatis -MCDA-AuNPs-LFB assay. Four reaction times ( A , 10 min; B , 20 min; C , 30 min; and D , 40 min) were evaluated at 67°C. Tubes/biosensors 1–7 represented C. trachomatis ompA template levels: 1.0 × 10 4 , 1.0 × 10 3 , 1.0 × 10 2 , 1.0 × 10 1 , 1.0 × 10 0 , 1.0 × 10 −1 copies, and negative control (distilled water, DW), respectively. Results were simultaneously analyzed using malachite green (MG) and AuNP-LFB. The optimal limit of detection (LoD) occurred when the amplification lasted for 30 min (C) . CL: control line; TL: test line.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Sensitive and visual identification of Chlamydia trachomatis using multiple cross displacement amplification integrated with a gold nanoparticle-based lateral flow biosensor for point-of-care use

doi: 10.3389/fcimb.2022.949514

Figure Lengend Snippet: Optimal amplification time for the C. trachomatis -MCDA-AuNPs-LFB assay. Four reaction times ( A , 10 min; B , 20 min; C , 30 min; and D , 40 min) were evaluated at 67°C. Tubes/biosensors 1–7 represented C. trachomatis ompA template levels: 1.0 × 10 4 , 1.0 × 10 3 , 1.0 × 10 2 , 1.0 × 10 1 , 1.0 × 10 0 , 1.0 × 10 −1 copies, and negative control (distilled water, DW), respectively. Results were simultaneously analyzed using malachite green (MG) and AuNP-LFB. The optimal limit of detection (LoD) occurred when the amplification lasted for 30 min (C) . CL: control line; TL: test line.

Article Snippet: Using 135 suspected C. trachomatis -infected genital secretion samples from Hangzhou Women’s Hospital (Hangzhou, China), we compared our assay with a commercial C. trachomatis real-time TaqMan PCR method (DaAn Gene Co., Ltd. China) (Cat. #DA0071) on an Applied BiosystemsTM 7500 Real-Time PCR System (Life Technologies, Singapore), which was used as a reference method since it is commonly used in clinical Chinese laboratories, and its sensitivity was verified using C. trachomatis standard substance (Guangzhou BDS Biological Technology Co., Ltd.).

Techniques: Amplification, Negative Control, Control

Analytical specificity of the C. trachomatis -MCDA-AuNPs-LFB assay using different strains. Assay specificity was evaluated using different nucleic acids as temperatures, and products were tested using AuNPs-LFB. Biosensors 1–14, C. trachomatis serovars A, B, C, D, E, F, G, H, I, J, K, L1, L2, and L3 ompA -plasmids; Biosensors 15–21, C. trachomatis (clinical samples); Biosensor 22, Ureaplasma urealyticum ; Biosensor 23, Neisseria gonorrhoeae ; Biosensor 24, Escherichia coli ; Biosensor 25, Staphylococcus aureus ; Biosensor 26, Human papilloma virus; Biosensor 27, Human rhinovirus; Biosensor 28, Coxsackie virus CAV16; Biosensor 29, Human enterovirus EV71; Biosensor 30, Mycoplasma pneumoniae ; Biosensor 31, Listeria monocytogenes ; Biosensor 32, Haemophilus influenza ; Biosensor 33, Cryptococcus neoformans ; Biosensor 34, Bordetella pertussis ; Biosensor 35, Streptococcus pyogenes ; Biosensor 36, Candida glabrata ; Biosensor 37, Pseudomonas aeruginosa ; Biosensor 38, Shigella flexneri ; Biosensor 39, Klebsiella pneumoniae ; Biosensor 40, negative control (distilled water, DW). CL: control line; TL: test line.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Sensitive and visual identification of Chlamydia trachomatis using multiple cross displacement amplification integrated with a gold nanoparticle-based lateral flow biosensor for point-of-care use

doi: 10.3389/fcimb.2022.949514

Figure Lengend Snippet: Analytical specificity of the C. trachomatis -MCDA-AuNPs-LFB assay using different strains. Assay specificity was evaluated using different nucleic acids as temperatures, and products were tested using AuNPs-LFB. Biosensors 1–14, C. trachomatis serovars A, B, C, D, E, F, G, H, I, J, K, L1, L2, and L3 ompA -plasmids; Biosensors 15–21, C. trachomatis (clinical samples); Biosensor 22, Ureaplasma urealyticum ; Biosensor 23, Neisseria gonorrhoeae ; Biosensor 24, Escherichia coli ; Biosensor 25, Staphylococcus aureus ; Biosensor 26, Human papilloma virus; Biosensor 27, Human rhinovirus; Biosensor 28, Coxsackie virus CAV16; Biosensor 29, Human enterovirus EV71; Biosensor 30, Mycoplasma pneumoniae ; Biosensor 31, Listeria monocytogenes ; Biosensor 32, Haemophilus influenza ; Biosensor 33, Cryptococcus neoformans ; Biosensor 34, Bordetella pertussis ; Biosensor 35, Streptococcus pyogenes ; Biosensor 36, Candida glabrata ; Biosensor 37, Pseudomonas aeruginosa ; Biosensor 38, Shigella flexneri ; Biosensor 39, Klebsiella pneumoniae ; Biosensor 40, negative control (distilled water, DW). CL: control line; TL: test line.

Article Snippet: Using 135 suspected C. trachomatis -infected genital secretion samples from Hangzhou Women’s Hospital (Hangzhou, China), we compared our assay with a commercial C. trachomatis real-time TaqMan PCR method (DaAn Gene Co., Ltd. China) (Cat. #DA0071) on an Applied BiosystemsTM 7500 Real-Time PCR System (Life Technologies, Singapore), which was used as a reference method since it is commonly used in clinical Chinese laboratories, and its sensitivity was verified using C. trachomatis standard substance (Guangzhou BDS Biological Technology Co., Ltd.).

Techniques: Virus, Negative Control, Control

Comparing C. trachomatis levels in clinical samples using our MCDA-AuNPs-LFB assay with a qPCR method.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Sensitive and visual identification of Chlamydia trachomatis using multiple cross displacement amplification integrated with a gold nanoparticle-based lateral flow biosensor for point-of-care use

doi: 10.3389/fcimb.2022.949514

Figure Lengend Snippet: Comparing C. trachomatis levels in clinical samples using our MCDA-AuNPs-LFB assay with a qPCR method.

Article Snippet: Using 135 suspected C. trachomatis -infected genital secretion samples from Hangzhou Women’s Hospital (Hangzhou, China), we compared our assay with a commercial C. trachomatis real-time TaqMan PCR method (DaAn Gene Co., Ltd. China) (Cat. #DA0071) on an Applied BiosystemsTM 7500 Real-Time PCR System (Life Technologies, Singapore), which was used as a reference method since it is commonly used in clinical Chinese laboratories, and its sensitivity was verified using C. trachomatis standard substance (Guangzhou BDS Biological Technology Co., Ltd.).

Techniques: